"sandwich hybridization assay"

Critical comparison of molecular methods for detection and enumeration of the harmful algal species, Heterosigma akashiwo, in environmental water samples

Molecular methods such as quantitative real-time PCR (qPCR) and sandwich hybridization assay (SHA) enable a more rapid and specific enumeration of harmful algal species compared to microscopic cell counts. Integrating these methods into routine …

Effects of growth phase, diel cycle and macronutrient stress on the quantification of Heterosigma akashiwo using qPCR and SHA

The development of molecular probe technologies over the last several decades has enabled more rapid and specific identification and enumeration of phytoplankton species compared to traditional technologies, such as light microscopy. Direct …

Comparison of sandwich hybridization assay and quantitative PCR for the quantification of live and preserved cultures of Heterosigma akashiwo (Raphidophyceae)

In this study, we directly compared two molecular techniques, sandwich hybridization assay (SHA) and quantitative PCR (qPCR), for quantifying laboratory cultures of the ichthyotoxic raphidophyte Heterosigma akashiwo. To maximize comparisons, all …